Recombinant Cynomolgus IL2RB protein, His-tagged, Biotinylated

• Cat.No.: IL2RB-619C
• Product Overview: Biotinylated Recombinant Cynomolgus IL2RB protein(Q38J85)(Ala27-Asp239), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 1-239 a.a.
• Tag: C-His
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.148 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.