Active Recombinant Cynomolgus IL4 protein, His-tagged, Low Endotoxin

• Cat.No.: IL4-1512C
• Product Overview: Recombinant Cynomolgus IL4 protein(P79339-1)(25-153 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 25-153 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus IL-4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 0.88ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 20-28 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.