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Active Recombinant Cynomolgus Monkey TIMD4 protein, Fc-tagged

Cat.No. : TIMD4-198C
Product Overview : Recombinant Cynomolgus Monkey TIMD4(Met22-Pro142) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.
  • Specification
  • Gene Information
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Description : TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is an approximately 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain. Sequence alignment with the human TIM-4 shows that mature cynomolgus monkey TIM-4 has a predicted 280 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 43 aa cytoplasmic tail. Within the ECD, cynomolgus monkey TIM-4 shares 87 % and 48 % aa sequence identity with human and mouse TIM-4, respectively. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1or TIM-3. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells.
Source : HEK293
Species : Cynomolgus Monkey
Tag : Fc
Predicted N Terminal : Glu25
Form : Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Bio-activity : Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.2-1.2 μg/mL.
Molecular Mass : Predicted Molecular Mass: 57 kDa
SDS-PAGE: 95-112 kDa, reducing conditions
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
Gene Name : TIMD4 T-cell immunoglobulin and mucin domain containing 4 [ Macaca fascicularis ]
Official Symbol : TIMD4
Synonyms : TIMD4; T-cell immunoglobulin and mucin domain containing 4
Gene ID : 102140672

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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