|Product Overview:||Recombinant Cynomolgus SIRPA Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 370 (Accession # NP_001271679.1). Predicted N-terminus: Glu 31. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.0 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus SIRPA Protein, His-tagged with a linear range of 0.313-2.5 μg/mL (QC tested).
Immobilized Biotinylated Human CD47, Fc Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2ug/well) plate, can bind Recombinant Cynomolgus SIRPA Protein, His-tagged with a linear range of 0.039-0.625 μg/mL (Routinely tested).
|Molecular Mass:||39.0 kDa|
|Protein length:||Glu 31 - Asn 370|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|