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Active Recombinant Cynomolgus SIRPA protein, His-tagged

Cat.No. : SIRPA-343C
Product Overview : Recombinant Cynomolgus SIRPA protein(Glu 31 - Asn 370 ), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Cynomolgus
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-1.25 μg/mL. Immobilized Biotinylated Human CD47, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2ug/well) plate, can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-0.625 μg/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Glu 31 - Asn 370
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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09/30/2020

    I am thankful for the immense support provided by this protein reagent for my experimental work! 

    06/14/2020

      This protein substance is truly an indispensable tool in my laboratory!

      09/03/2019

        Its performance is stable and reliable, and the manufacturer's quality control ensures that every experiment runs smoothly.

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