Recombinant Cynomolgus SIRPA protein(Met1-Tyr372), His-tagged

• Cat.No.: SIRPA-1158C
• Product Overview: Recombinant Cynomolgus SIRPA (XP_015313153.1) (Met1-Tyr372) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Met1-Tyr372
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized cynoSIRPAh at 10 μg/mL (100 μL/well) can bind CD47-Fc, the EC50 of CD47-Fc is 3.0-10.0 ng/mL.
• Molecular Mass: The recombinant cynomolgus SIRPA comprises 353 amino acids and has a calculated molecular mass of 38.93 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.