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Recombinant Cynomolgus TP53 Protein

Cat.No.: TP53-1162C
Product Overview: Recombinant Cynomolgus (Macaca fascicularis) p53 (E3U906) (Met 1-Asp 393) was expressed and purified.
Source: E. coli
Species: Cynomolgus
Predicted N Terminal: Met 1
Form: Lyophilized from sterile 100mM Tris, 3% glycerol, 4mM DTT, pH 8.01, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to bind biotinylated human S100B in a functional ELISA. 2. Measured by its ability to bind biotinylated human RRM2B in a functional ELISA.
Molecular Mass: The recombinant Cynomolgus p53 consisting of 393 amino acids and has a calculated molecular mass of 43.7 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >88 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TP53 tumor protein p53 [ Macaca fascicularis ]
Official Symbol: TP53
Synonyms: p53; Tumor suppressor p53
Gene ID: 102135998
mRNA Refseq: NM_001287679
Protein Refseq: NP_001274608
MIM:
UniProt ID: P56423
Chromosome Location: chromosome: 16
Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Bladder cancer, organism-specific biosystem; Central carbon metabolism in cancer, conserved biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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