Recombinant Cynomolgus TSLP protein, His-tagged, Biotinylated, Primary Amine Labeling

• Cat.No.: TSLP-5742C
• Product Overview: Primary Amine Labeling Biotinylated Recombinant Cynomolgus TSLP protein(A0A2K5TXV0-1)(Tyr29-Gln159), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Tyr29-Gln159
• Tag: C-His
• Conjugation/Label: Biotin; Primary Amine Labeling
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Cynomolgus TSLP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 23-26 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.