Source : |
HEK293 |
Species : |
Cynomolgus |
Tag : |
His |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus TSLP (R127A, R130S) Protein, His Tag with a linear range of 1-4 ng/mL. Immobilized Cynomolgus TSLP (R127A, R130S) Protein, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with a linear range of 0.5-8 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 16.9 kDa. The protein migrates as 21-30 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
Tyr29-Gln159(R127A,R130S) |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |