Active Recombinant Dog CYP2C21 Protein

• Cat.No.: CYP2C21-114D
• Product Overview: Recombinant dog CYP2C21 protein without tag were coexpressed in E. coli.

Specification

• Species: Dog
• Source: E.coli
• Description: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Showed testosterone hydrolase activity.
• Bio-activity: Cytochrome c Reductase Activity: 327 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Diclofenac 4’-hydroxylase
  Linear with time up to: 12 min(Determined at 30 µM substrate, using a P450 concentration of 100 pmol/ml)
  Linear with P450 up to: 125 pmol/ml(Determined at 30 µM substrate, using an incubation time of 5 min)
  Vmax: 1.7 pmol/min/pmol CYP
  Km: 57 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 3.1 nmol/ml
  Protein Concentration: 16.2 mg/ml
  Specific P450 Content: 191 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C18 cytochrome P450 2C21 [ Canis lupus familiaris (dog) ]
• Official Symbol: CYP2C21
• Synonyms: CYP2C21; cytochrome P450 2C21; CYP2C19; CYP2C21; CYPIIC21; cytochrome P450 2C21; Cytochrome-P450-2C21; cytochrome P450 DM1-1
• Gene ID: 415122
• mRNA Refseq: NM_001197044
• Protein Refseq: NP_001183973
• UniProt ID: P56594