Active Recombinant Dog CYP2C41 Protein

• Cat.No.: CYP2C41-115D
• Product Overview: Recombinant dog CYP2C41 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
• Source: E. coli
• Species: Dog
• Bio-activity: Cytochrome c Reductase Activity: 608 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Diclofenac 4’-hydroxylase
  Linear with time up to: 60 min(Determined at 500 µM substrate, using a P450 concentration of 150 pmol/ml)
  Linear with P450 up to: 200 pmol/ml(Determined at 500 µM substrate, using an incubation time of 30 min)
  Vmax: 0.27 pmol/min/pmol CYP
  Km: 60 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 7.6 nmol/ml
  Protein Concentration: 26.0 mg/ml
  Specific P450 Content: 292 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2C41 cytochrome P450 2C41 [ Canis lupus familiaris (dog) ]
• Official Symbol: CYP2C41
• Synonyms: CYP2C41; cytochrome P450 2C41; CYPIIC41; cytochrome P450 2C41; cytochrome P450, family 2, subfamily C, polypeptide 41
• Gene ID: 415123
• mRNA Refseq: NM_001003334
• Protein Refseq: NP_001003334
• UniProt ID: O62671