Recombinant Dog CYP2D15 Protein

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Recombinant Dog CYP2D15 Protein

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Cat.No. : CYP2D15-116D
Product Overview : Recombinant dog CYP2D15 protein without tag were coexpressed in E. coli.
Description : High activity for the hydroxylation of bunitrolol and imipramine; low activity on debrisoquine.
Source : E. coli
Species : Dog
Bio-activity : Cytochrome c Reductase Activity: 330 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Bufuralol 1’-hydroxylase
Linear with time up to: 8 min(Determined at 3 µM substrate, using a P450 concentration of 5 pmol/ml)
Linear with P450 up to: 15 pmol/ml(Determined at 3 µM substrate, using an incubation time of 5 min)
Vmax: 5.4 pmol/min/pmol CYP
Km: 6.2 μM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 5.2 nmol/ml
Protein Concentration: 21.2 mg/ml
Specific P450 Content: 245 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP2D15 cytochrome P450 2D [ Canis lupus familiaris (dog) ]
Official Symbol : CYP2D15
Synonyms : CYP2D15; cytochrome P450 2D; CYP2D; CYPIID15; cytochrome P450 2D15; Cytochrome-P450-2D15; cytochrome P450 DUT2; cytochrome P450, family 2, subfamily D, polypeptide 15; monooxygenase
Gene ID : 415120
mRNA Refseq : NM_001003333
Protein Refseq : NP_001003333
UniProt ID : Q29473

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