Recombinant Dog KIT Protein, His-tagged

• Cat.No.: KIT-2364D
• Product Overview: Recombinant Dog KIT Protein (Gln28-Pro527) with a N-His tag was expressed in E. coli.

Specification

• Description: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
• Source: E. coli
• Species: Dog
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.7 kDa
  Accurate Molecular Mass: 57 kDa
• Protein length: Gln28-Pro527
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: KIT KIT proto-oncogene, receptor tyrosine kinase [ Canis lupus familiaris (dog) ]
• Official Symbol: KIT
• Synonyms: KIT; KIT proto-oncogene, receptor tyrosine kinase; PBT; SCFR; C-Kit; CD117; MASTC; mast/stem cell growth factor receptor Kit
  c-Kit protooncogene
  p145 c-kit
  piebald trait protein
  proto-oncogene c-Kit
  proto-oncogene tyrosine-protein kinase Kit
  tyrosine-protein kinase Kit
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
  EC 2.7.10.1
• Gene ID: 403811
• mRNA Refseq: NM_001003181
• Protein Refseq: NP_001003181
• UniProt ID: O97799