Recombinant Drosophila Melanogaster Trithorax-like, Isoform A

• Cat.No.: Trl-352D
• Product Overview: Recombinant Drosophila melanogasterGAGA-POZ domain was overexpressed in E.coli and purified by using conventional chromatography techniques, 14 kDa (130 aa).

Specification

• Species: Drosophila
• Source: E.coli
• Tag: Non
• Description: The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo.
• Form: Liquid. In 10 mM HEPES (pH 7.4) containing 25 mM NaCl
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFVYRGE VSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: Trl Trithorax-like [ Drosophila melanogaster ]
• Synonyms: Adf-2; Adf-2-519; Adf2; CG9343; E(var)3-trl; E(var)62; GAF; GAGA; NC70F; TRL; TfGAGA/Adf-2; Trl-GAGA; anon-EST:fe2E12; l(3)s2325; Dmel\CG33261; CG33261; Trl; Trithorax-like, isoform B; CG33261-PB; Trl-PB; GAGA transcription factor; anon-fast-evolving-2E12; GAGA-factor; GAGA factor; transcription-factor-Adh2; Adh transcription factor 2; neural conserved at 70F; TRITHORAX-LIKE; GAGA element; Trithorax-like, isoform I; CG33261-PI; Trl-PI; Trithorax-like, isoform D; CG33261-PD; Trl-PD; Trithorax-like, isoform A; CG33261-PE; Trl-PE.
• Gene ID: 2768981
• mRNA Refseq: NM_206358
• Protein Refseq: NP_996080
• UniProt ID: Q08605
• Function: DNA binding; DNA binding; RNA polymerase II transcription factor activity; protein binding; specific RNA polymerase II transcription factor activity; specific RNA polymerase II transcription factor activity; zinc ion binding.

Citation

Tandem mass spectrometry analysis of urinary podocalyxin and podocin in the investigation of podocyturia in women with preeclampsia and Fabry disease patients.

Journal: Clinica chimica acta; international journal of clinical chemistry    PubMed ID: 30898510    Data: 2019/12/2

Authors: Tristan Martineau, Michel Boutin, Christiane Auray-Blais

Article Snippet:Recombinant podocin (NPHS2, > 80%) was from Novus Biologicals while recombinant podocalyxin isoform 2 (PODXL, > 80%) was from Creative BioMart. . JPT Peptide Technologies Gmbh synthesized the stable isotope-labelled (SIL) peptides (APAATVVDVDEVR?; ATFNPAQDK?), the cleavable stable isotope-labelled (CSIL) peptides (CRAVK/ ATFNPAQDK?/CGIRL; PGEPR/APAATVVDVDEVR?/GSGEE) and their unlabelled counterparts (APAATVVDVDEVR; ATFNPAQDK; CRAVK/ ATFNPAQDK/CGIRL; PGEPR/APAATVVDVDEVR/GSGEE).JPT Peptide Technologies Gmbh synthesized the stable isotope-labelled (SIL) peptides (APAATVVDVDEVR?; ATFNPAQDK?), the cleavable stable isotope-labelled (CSIL) peptides (CRAVK/ ATFNPAQDK?/CGIRL; PGEPR/APAATVVDVDEVR?/GSGEE) and their unlabelled counterparts (APAATVVDVDEVR; ATFNPAQDK; CRAVK/ ATFNPAQDK/CGIRL; PGEPR/APAATVVDVDEVR/GSGEE).