Recombinant E.coli dsbA protein

• Cat.No.: Dsf-525H
• Product Overview: Recombinant DsbA (residues 20-208) was expressed inE.coliand purified by using conventional chromatography techniques. MW =21.1kDa (189aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 20-208 a.a.
• Description: Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.coli is a periplasmic protein and thioredoxin superfamily member whichintroduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids).
• Amino Acid Sequence: AQYEDGKQYT TLEKPVAGAP QVLEFFSFFC PHCYQFEEVL HISDNVKKKL PEGVKMTKYH VNFMGGDLGK DLTQAWAVAM ALGVEDKVTVPLFEGVQKTQ TIRSASDIRD VFINAGIKGE EYDAAWNSFV VKSLVAQQEK AAADVQLRGV PAMFVNGKYQ LNPQGMDTSN MDVFVQQYADTVKYLSEKK.
• Form: Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 2 mM EDTA.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: dsbA periplasmic protein disulfide isomerase I [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: dsbA; Disulfide Oxidoreductase A; dsf; ECK3852; iarA; JW3832; ppfA; periplasmic protein disulfide isomerase I; Thiol:disulfide interchange protein dsbA
• Gene ID: 948353
• Protein Refseq: NP_418297
• UniProt ID: P0AEG4