Recombinant E. coli mSA2 Protein, N-His/C-FLAG-tagged

• Cat.No.: mSA2-36E
• Product Overview: Recombinant E. coli mSA Protein, fused to His-tag at N-terminusand and FLAG-tag at C-terminus, was expressed in E. coli.

Specification

• Species: E.coli
• Source: E.coli
• Tag: Flag&His
• Description: Monomeric Streptavidin 2 (mSA2) is an engineered monomeric streptavidin protein that binds biotin with Kd ~ 2 – 3 nM. The monomer was designed by homology modeling, in which the streptavidin and rhizavidin sequences were combined to engineer a high affinity binding pocket containing residues from a single subunit only.
• Form: 50 mM Tris (pH 7.5), 150 mM NaCl.
• Molecular Mass: 15.6 kDa (including FLAG tag)
• AA Sequence: TSHHHHHHEFASAEAGITGTWYNQHGSTFTVTAGADGNLTGQYENRAQGTGCQNSPYTLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATEQGQDTFTKVKPSAASGSDYKDDDDK
• Purity: >90% by SDS-PAGE
• Storage: Store at 4 centigrade up to 12 months. Flash freeze in liquid nitrogen and place at -80 centigradefor long-term storage. Avoid repeated freeze-thaw cycles.
• Dilutions: >30mg/mL in PBS.
• Shipping: Cold packs

Gene Information

• Gene Name: mSA2
• UniProt ID: P22629