Recombinant E. coli Tol2 protein, His-tagged

• Cat.No.: tolA-01E
• Product Overview: Recombinant protein of tolA was expressed in E. coli, with a His-Tag.

Specification

• Description: Cell envelope integrity is stabilized by the Tol-Pal system linking inner and outer membranes. TolA is one of three (along with TolQ and TolR) inner membrane protein members of the YbgC-YbgF-TolQ-R-A-B-Pal Cell Envelope Complex, also known as the Tol-Pal system.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in 10 mM HEPES, 300 mM KCl, pH 6.9.
• Molecular Mass: ~74kDa
• AA Sequence: MHHHHHHEEVCDSSAAASSTVQNQP QDQEHPWPYLREFFSLSGVNKDSFK MKCVLCLPLNKEISAFKSSPSNLRK HIERMHPNYLKNYSKLTAQKRKIGT STHASSSKQLKVDSVFPVKHVSPVT VNKAILRYIIQGLHPFSTVDLPSFK ELISTLQPGISVITRPTLRSKIAEA ALIMKQKVTAAMSEVEWIATTTDCW TARRKSFIGVTAHWINPGSLERHSA ALACKRLMGSHTFEVLASAMNDIHS EYEIRDKVVCTTTDSGSNFMKAFRV FGVENNDIETEARRCESDDTDSEGC GEGSDGVEFQDASRVLDQDDGFEFQ LPKHQKCACHLLNLVSSVDAQKALS NEHYKKLYRSVFGKCQALWNKSSRS ALAAEAVESESRLQLLRPNQTRWNS TFMAVDRILQICKEAGEGALRNICT SLEVPMFNPAEMLFLTEWANTMRPV AKVLDILQAETNTQLGWLLPSVHQL SLKLQRLHHSLRYCDPLVDALQQGI QTRFKHMFEDPEIIAAAILLPKFRT SWTNDETIIKRGMDYIRVHLEPLDH KKELANSSSDDEDFFASLKPTTHEA SKELDGYLACVSDTRESLLTFPAIC SLSIKTNTPLPASAACERLFSTAGL LFSPKRARLDTNNFENQLLLKLNLR FYNFE
• Endotoxin: < 1 EU/ug by the LAL method
• Purity: >90% as determined by SDS-PAGE
• Storage: Short Term Storage at 4 centigrade, Long Term, please prepare aliquots with 20% glycerol and store at -20 to -80 centigrade. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: tolA Tol-Pal system protein TolA [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: tolA
• Synonyms: cim; ECK0728; excC; lky; tol-2
• Gene ID: 946625
• Protein Refseq: NP_415267
• UniProt ID: P19934