Recombinant EBV chimeric early antigen, His&TrxA tagged

• Cat.No.: EA-086E
• Product Overview: The recombinant chimeric early antigen ChimEBV-EA for Epstein-Barr Virus (EBV) has been prepared as a recombinant multi-epitope antigen fused to a his-tag and trxA at the N-terminal

Specification

• Species: EBV
• Source: E.coli
• Tag: His&TRxA
• Tag: N-His&TrxA
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 45-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 54,319.48 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.53 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Neuhierl, B. and Henri-Jacques Delecluse, H.J. The Epstein-Barr Virus BMRF1 Gene Is Essential for Lytic Virus Replication. Journal of Virology, 2006, Vol. 80, No. 10: 5078-5081.
  2. Mumtsidu E, Makhov AM, Konarev PV, Svergun DI, Griffith JD, Tucker PA. Structural features of the single-stranded DNA-binding protein of Epstein-Barr virus. 2008 J. Struct. Biol. 161(2):172-87.
  3. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.