Recombinant E.coli idnk Protein, His-tagged
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| Cat.No. : | idnk-394E |
| Product Overview : | Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Description : | D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. |
| Source : | E.coli |
| Species : | E.coli |
| Tag : | His |
| Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Bio-activity : | Specific activity is >80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C. |
| Molecular Mass : | 23.4 kDa (210aa), confirmed by MALDI-TOF |
| AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDASYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE |
| Purity : | >90% by SDS - PAGE |
| Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : | 1 mg/ml (determined by Bradford assay) |
| Gene Name : | idnK D-gluconate kinase, thermosensitive [ Escherichia coli str. K-12 substr. MG1655 ] |
| Official Symbol : | idnk |
| Synonyms : | ECK4261; gntV; JW4225; D-gluconate kinase, thermosensitive |
| Gene ID : | 946066 |
| mRNA Refseq : | |
| Protein Refseq : | NP_418689 |
| MIM : | |
| UniProt ID : | P39208 |
| Pathway : | Biosynthesis of antibiotics, organism-specific biosystem; Biosynthesis of secondary metabolites, organism-specific biosystem; L-idonate degradation, conserved biosystem |
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