Recombinant E.coli idnk Protein, His-tagged idnk-394E

Recombinant E.coli idnk Protein, His-tagged

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Recombinant E.coli idnk Protein, His-tagged

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Cat.No. : idnk-394E
Product Overview : Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype.
Source : E.coli
Species : E.coli
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Bio-activity : Specific activity is >80 units/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.
Molecular Mass : 23.4 kDa (210aa), confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDASYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
Purity : >90% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : idnK D-gluconate kinase, thermosensitive [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : idnk
Synonyms : ECK4261; gntV; JW4225; D-gluconate kinase, thermosensitive
Gene ID : 946066
mRNA Refseq :
Protein Refseq : NP_418689
MIM :
UniProt ID : P39208
Pathway : Biosynthesis of antibiotics, organism-specific biosystem; Biosynthesis of secondary metabolites, organism-specific biosystem; L-idonate degradation, conserved biosystem

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