Active Recombinant Entamoeba histolytica ADH1 protein, His-tagged

• Cat.No.: ADH1-156
• Product Overview: Recombinant Entamoeba histolytica ADH1 fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2-ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.
• Source: E. coli
• Species: Entamoeba histolytica
• Tag: His
• Form: 1 mg/mL solution in 50 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 50% glycerol
• Bio-activity: This NADP-dependent isopropanol dehydrogenase has biological activity ≥60U/mg.
• Molecular Mass: ~40.9 kDa
• Protein length: 360 amino acids
• Purity: Purity by SDS-PAGE: ≥95%
• Unit Definition: One unit is the amount of enzyme that will generate 1.0 µmole of NADPH per minute at pH 8 at 37 °C.
• Notes: Centrifuge the vial prior to opening
• Storage: Storage at -20 centigrade