Recombinant Escherichia coli NFSA Protein (1-240 aa), His-tagged

• Cat.No.: NFSA-1585E
• Product Overview: Recombinant Escherichia coli (strain K12) NFSA Protein (1-240 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Species: E.coli
• Source: Yeast
• Tag: His
• Protein Length: 1-240 aa
• Description: Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E.coli.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 28.8 kDa
• AA Sequence: MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: nfsA NADPH-dependent nitroreductase NfsA [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: NFSA
• Synonyms: ECK0842; mda18; mdaA; ybjB
• Gene ID: 945483
• Protein Refseq: NP_415372
• UniProt ID: P17117