Recombinant Escherichia coli RTCB Protein (1-408 aa), His-tagged

• Cat.No.: RTCB-1601E
• Product Overview: Recombinant Escherichia coli (strain K12) RTCB Protein (1-408 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: RNA ligase that mediates the joining of broken tRNA-like st-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like st-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester, a step that requires GTP . Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases.
• Source: Yeast
• Species: E. coli
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 47.2 kDa
• Protein length: 1-408 aa
• AA Sequence: MNYELLTTENAPVKMWTKGVPVEAD ARQQLINTAKMPFIFKHIAVMPDVH LGKGSTIGSVIPTKGAIIPAAVGVD IGCGMNALRTALTAEDLPENLAELR QAIETAVPHGRTTGRCKRDKGAWEN PPVNVDAKWAELEAGYQWLTQKYPR FLNTNNYKHLGTLGTGNHFIEICLD ESDQVWIMLHSGSRGIGNAIGTYFI DLAQKEMQETLETLPSRDLAYFMEG TEYFDDYLKAVAWAQLFASLNRDAM MENVVTALQSITQKTVRQPQTLAME EINCHHNYVQKEQHFGEEIYVTRKG AVSARAGQYGIIPGSMGAKSFIVRG LGNEESFCSCSHGAGRVMSRTKAKK LFSVEDQIRATAHVECRKDAEVIDE IPMAYKDIDAVMAAQSDLVEVIYTL RQVVCVKG
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: rtcB RNA-splicing ligase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: RTCB
• Synonyms: ECK3407; yhgL
• Gene ID: 947929
• Protein Refseq: NP_417879
• UniProt ID: P46850