Recombinant Full Length Human RTCB Protein, C-Flag-tagged

• Cat.No.: RTCB-1083HFL
• Product Overview: Recombinant Full Length Human RTCB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables RNA ligase (ATP) activity and vinculin binding activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytosol and nucleoplasm. Part of tRNA-splicing ligase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 55 kDa
• AA Sequence: MSRSYNDELQFLEKINKNCWRIKKG FVPNMQVEGVFYVNDALEKLMFEEL RNACRGGGVGGFLPAMKQIG NVAALPGIVHRSIGLPDVHSGYGFA IGNMAAFDMNDPEAVVSPGGVGFDI NCGVRLLRTNLDESDVQPVK EQLAQAMFDHIPVGVGSKGVIPMNA KDLEEALEMGVDWSLREGYAWAEDK EHCEEYGRMLQADPNKVSAR AKKRGLPQLGTLGAGNHYAEIQVVD EIFNEYAAKKMGIDHKGQVCVMIHS GSRGLGHQVATDALVAMEKA MKRDKIIVNDRQLACARIASPEGQD YLKGMAAAGNYAWVNRSSMTFLTRQ AFAKVFNTTPDDLDLHVIYD VSHNIAKVEQHVVDGKERTLLVHRK GSTRAFPPHHPLIAVDYQLTGQPVL IGGTMGTCSYVLTGTEQGMT ETFGTTCHGAGRALSRAKSRRNLDF QDVLDKLADMGIAIRVASPKLVMEE APESYKNVTDVVNTCHDAGI
  SKKAIKLRPIAVIKGTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: RTCB RNA 2',3'-cyclic phosphate and 5'-OH ligase [ Homo sapiens (human) ]
• Official Symbol: RTCB
• Synonyms: FAAP; HSPC117; C22orf28; DJ149A16.6
• Gene ID: 51493
• mRNA Refseq: NM_014306.5
• Protein Refseq: NP_055121.1
• MIM: 613901
• UniProt ID: Q9Y3I0