Recombinant Escherichia coli (strain UTI89/UPEC) clpP protein (ATP-dependent Clp protease proteolytic subunit), His/Myc-tagged

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Recombinant Escherichia coli (strain UTI89/UPEC) clpP protein (ATP-dependent Clp protease proteolytic subunit), His/Myc-tagged

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Cat.No. : CLPP-1032E
Product Overview : Recombinant Escherichia coli (strain UTI89/UPEC) clpP protein (ATP-dependent Clp protease proteolytic subunit, 15-207aa), fused to 10×His-tag at N-terminus and Myc-tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : ClpP can simultaneously bind ClpX and ClpA to form a heteromeric complex. ClpP is a serine protease with a chymotrypsin-like activity that is a part of the ClpAP, ClpAPX and ClpXP protease complexes. Cleaves peptides in various proteins in a process that requires ATP hydrolysis. The protein has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.
Source : E.coli
Species : E.coli
Tag : His/Myc
Form : Tris-based buffer,50% glycerol.
Molecular Mass : 28.6 kDa
Protein length : 193
AA Sequence : ALVPMVIEQT SRGERSFDIY SRLLKERVIF LTGQVEDHMA NLIVAQMLFL EAENPEKDIY LYINSPGGVI TAGMSIYDTM QFIKPDVSTI CMGQAASMGA FLLTAGAKGK RFCLPNSRVM IHQPLGGYQG QATDIEIHAR EILKVKGRMN ELMALHTGQS LEQIERDTER DRFLSAPEAV EYGLVDSILT HRN
Purity : Greater than 85% as determined by SDS-PAGE.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20/-80 centigrade. The shelf life of lyophilized form is 12 months at -20/-80 centigrade. Store working aliquots at 4 centigrade for up to one week.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Warning : Repeated freezing and thawing is not recommended.
Synonyms : ECK0431; lopP
UniProt ID : Q1RF98
SDS-PAGE of CLPP-1032E :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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