| Cat.No. : |
BBE1-1598E |
| Product Overview : |
Recombinant Eschscholzia Californica (California poppy) BBE1 Protein (24-538 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein. |
| Description : |
Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine. |
| Source : |
Yeast |
| Species : |
Eschscholzia Californica |
| Tag : |
His |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
59.4 kDa |
| Protein length : |
24-538 aa |
| AA Sequence : |
GNDLLSCLTF NGVRNHTVFS ADSDSDFNRF LHLSIQNPLF QNSLISKPSA IILPGSKEEL SNTIRCIRKG SWTIRLRSGG HSYEGLSYTS DTPFILIDLM NLNRVSIDLE SETAWVESGS TLGELYYAIT ESSSKLGFTA GWCPTVGTGG HISGGGFGMM SRKYGLAADN VVDAILIDAN GAILDRQAMG EDVFWAIRGG GGGVWGAIYA WKIKLLPVPE KVTVFRVTKN VAIDEATSLL HKWQFVAEEL EEDFTLSVLG GADEKQVWLT MLGFHFGLKT VAKSTFDLLF PELGLVEEDY LEMSWGESFA YLAGLETVSQ LNNRFLKFDE RAFKTKVDLT KEPLPSKAFY GLLERLSKEP NGFIALNGFG GQMSKISSDF TPFPHRSGTR LMVEYIVAWN QSEQKKKTEF LDWLEKVYEF MKPFVSKNPR LGYVNHIDLD LGGIDWGNKT VVNNAIEISR SWGESYFLSN YERLIRAKTL IDPNNVFNHP QSIPPMANFD YLEKTLGSDG GEVVI |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Synonyms : |
BBE1; Berberine bridge-forming enzyme; BBETetrahydroprotoberberine synthase; |
| UniProt ID : |
P30986 |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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