Recombinant Fire fly Luciferase

• Cat.No.: Luciferase-08F

Specification

• Cat. No.: Luciferase-08F
• Product overview: Recombinant Fire fly luciferase produced in E. coli has a molecular mass of approximately 62 KDa.
• Description: Luciferases are enzymes that emit light. A diverse group of organisms use luciferase-mediated bioluminescence to startle predators or to attract prey or mates. The luciferase from the North American fire fly releases green light during the oxidation of its chemical substrate, luciferin. Recombinant luciferase is expressed inE. coli.
• Purity: >95% as determined by SDS-PAGE.
• Specific Activity: more than 2.0×10¹¹RLU/mg.
• Physical Appearance: Lyophilized powder
• Optimum PH: 7.0—8.5
• PH Stability: 6.0—9.0
• Storage: This lyophilized preparation should be kept at –20°C for six months, and kept at –70°C for one year.
• Tag: Non
• Publication:
  Cry Protein Crystals: A Novel Platform for Protein Delivery (2015)
  Pore-like diffusion barriers in murine cardiac myocytes (2023)
  Longitudinal diffusion barriers imposed by myofilaments and mitochondria in murine cardiac myocytes (2023)

Reviews

05/18/2022

Worked well, happy customer.

08/30/2021

Great in lab experiments.

09/04/2020

Satisfied with the purchase.

Q&As

How is luciferase utilized in bioluminescence imaging?

It allows for real-time tracking of cellular and molecular processes in living organisms.

What is the primary function of luciferase in organisms that produce it?

Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

How does luciferase produce light in a biochemical reaction?

It catalyzes the oxidation of luciferin, releasing light.

In which organisms is luciferase commonly found?

Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

Are there different types of luciferase, and how do they vary?

Yes, there are different types, varying in their substrate specificity and the color of emitted light.

What is the role of ATP in the luciferase reaction?

ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

What advantages does the use of luciferase offer in scientific research?

Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.