Recombinant Full Length Human ACO1 Protein, C-Flag-tagged

• Cat.No.: ACO1-531HFL
• Product Overview: Recombinant Full Length Human ACO1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 98.2 kDa
• AA Sequence: MSNPFAHLAEPLDPVQPGKKFFNLN KLEDSRYGRLPFSIRVLLEAAIRNC DEFLVKKQDIENILHWNVTQ HKNIEVPFKPARVILQDFTGVPAVV DFAAMRDAVKKLGGDPEKINPVCPA DLVIDHSIQVDFNRRADSLQ KNQDLEFERNRERFEFLKWGSQAFH NMRIIPPGSGIIHQVNLEYLARVVF DQDGYYYPDSLVGTDSHTTM IDGLGILGWGVGGIEAEAVMLGQPI SMVLPQVIGYRLMGKPHPLVTSTDI VLTITKHLRQVGVVGKFVEF FGPGVAQLSIADRATIANMCPEYGA TAAFFPVDEVSITYLVQTGRDEEKL KYIKKYLQAVGMFRDFNDPS QDPDFTQVVELDLKTVVPCCSGPKR PQDKVAVSDMKKDFESCLGAKQGFK GFQVAPEHHNDHKTFIYDNT EFTLAHGSVVIAAITSCTNTSNPSV MLGAGLLAKKAVDAGLNVMPYIKTS LSPGSGVVTYYLQESGVMPY LSQLGFDVVGYGCMTCIGNSGPLPE PVVEAITQGDLVAVGVLSGNRNFEG RVHPNTRANYLASPPLVIAY AIAGTIRIDFEKEPLGVNAKGQQVF LKDIWPTRDEIQAVERQYVIPGMFK EVYQKIETVNESWNALATPS DKLFFWNSKSTYIKSPPFFENLTLD LQPPKSIVDAYVLLNLGDSVTTDHI SPAGNIARNSPAARYLTNRG LTPREFNSYGSRRGNDAVMARGTFA NIRLLNRFLNKQAPQTIHLPSGEIL DVFDAAERYQQAGLPLIVLA GKEYGAGSSRDWAAKGPFLLGIKAV LAESYERIHRSNLVGMGVIPLEYLP GENADALGLTGQERYTIIIP
  ENLKPQMKVQVKLDTGKTFQAVMRF DTDVELTYFLNGGILNYMIRKMAKT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways

Gene Information

• Gene Name: ACO1 aconitase 1 [ Homo sapiens (human) ]
• Official Symbol: ACO1
• Synonyms: IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
• Gene ID: 48
• mRNA Refseq: NM_002197.3
• Protein Refseq: NP_002188.1
• MIM: 100880
• UniProt ID: P21399