Recombinant Full Length Human ADSS2 Protein, C-Flag-tagged

• Cat.No.: ADSS2-266HFL
• Product Overview: Recombinant Full Length Human ADSS2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the enzyme adenylosuccinate synthetase which catalyzes the first committed step in the conversion of inosine monophosphate to adenosine monophosphate. A pseudogene of this gene is found on chromosome 17.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 49.9 kDa
• AA Sequence: MAFAETYPAASSLPNGDCGRPRARP GGNRVTVVLGAQWGDEGKGKVVDLL AQDADIVCRCQGGNNAGHTV VVDSVEYDFHLLPSGIINPNVTAFI GNGVVIHLPGLFEEAEKNVQKGKGL EGWEKRLIISDRAHIVFDFH QAADGIQEQQRQEQAGKNLGTTKKG IGPVYSSKAARSGLRMCDLVSDFDG FSERFKVLANQYKSIYPTLE IDIEGELQKLKGYMEKIKPMVRDGV YFLYEALHGPPKKILVEGANAALLD IDFGTYPFVTSSNCTVGGVC TGLGMPPQNVGEVYGVVKAYTTRVG IGAFPTEQDNEIGELLQTRGREFGV TTGRKRRCGWLDLVLLKYAH MINGFTALALTKLDILDMFTEIKVG VAYKLDGEIIPHIPANQEVLNKVEV QYKTLPGWNTDISNARAFKE
  LPVNAQNYVRFIEDELQIPVKWIGV GKSRESMIQLFTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

Gene Information

• Gene Name: ADSS2 adenylosuccinate synthase 2 [ Homo sapiens (human) ]
• Official Symbol: ADSS2
• Synonyms: ADEH; ADSS
• Gene ID: 159
• mRNA Refseq: NM_001126.5
• Protein Refseq: NP_001117.2
• MIM: 103060
• UniProt ID: P30520

Reviews

04/24/2019

Understanding protein-protein interaction networks in immune cell signaling.

01/23/2019

Exploring protein-protein interactions involved in neurodegenerative diseases.

07/04/2017

Detecting protein-protein interactions in cytoskeletal organization.

Q&As

What are the downstream metabolic pathways or cellular processes influenced by ADSS2 protein activity?

ADSS2 protein plays a critical role in the de novo synthesis of purine nucleotides, influencing downstream metabolic pathways involved in nucleotide and energy metabolism.

What are the effects of ADSS2 protein knockout or overexpression on cellular metabolism and physiological processes?

ADSS2 protein knockout or overexpression studies reveal significant impacts on cellular metabolism, particularly in purine nucleotide synthesis and related physiological processes such as cell proliferation and energy homeostasis.

What are the enzymatic assays or substrate/product measurements used to assess the activity of ADSS2 protein?

Enzymatic activity of ADSS2 protein is measured using assays that quantify the conversion of substrates or the production of specific products involved in purine nucleotide synthesis.

Are there any known post-translational modifications of ADSS2 protein, and how do they affect its enzymatic activity or stability?

ADSS2 protein undergoes phosphorylation and acetylation post-translational modifications, which can impact its enzymatic activity and stability.

Are there any specific signaling pathways or transcription factors that regulate the expression of ADSS2 protein?

The expression of ADSS2 protein can be regulated by various signaling pathways, including those activated by growth factors or stress stimuli, and transcription factors such as c-Myc or HIF-1伪.

What experimental techniques have been used to investigate the tissue-specific expression pattern of ADSS2 protein?

Immunohistochemistry and Western blotting have been employed to investigate the tissue-specific expression pattern of ADSS2 protein.

Can ADSS2 protein form protein complexes or interact with other proteins, and if so, what are the functional implications?

ADSS2 protein has the potential to interact with other proteins, including enzymes involved in purine metabolism, which can modulate its function and cellular processes.

Are there any genetic variations or mutations in the ADSS2 gene associated with changes in protein function or disease susceptibility?

Genetic variations or mutations in the ADSS2 gene can lead to alterations in protein function or stability, potentially influencing purine metabolism disorders or disease susceptibility.

How is the subcellular localization of ADSS2 protein regulated, and does it vary in different cellular contexts or under specific conditions?

The subcellular localization of ADSS2 protein is predominantly cytoplasmic, but it may undergo changes in response to cellular signals or developmental cues.

How does ADSS2 protein contribute to cellular growth, proliferation, or differentiation?

ADSS2 protein contributes to cellular growth, proliferation, and differentiation by providing the necessary purine nucleotides for DNA and RNA synthesis.