Recombinant Full Length Human AHSG Protein, GST-tagged

• Cat.No.: AHSG-952HF
• Product Overview: Human AHSG full-length ORF ( AAH48198.1, 19 a.a. - 367 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 64.13 kDa
• Protein length: 367 amino acids
• AA Sequence: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VLLAAPPGHQ LHRAHYDLRH TFMGVVSLGS PSGEVSHPRK TRTVVQPSVG AAAGPVVPPC PGRIRHFKV
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AHSG alpha-2-HS-glycoprotein [ Homo sapiens ]
• Official Symbol: AHSG
• Synonyms: AHSG; alpha-2-HS-glycoprotein; A2HS; FETUA; HSGA; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein; AHS
• Gene ID: 197
• mRNA Refseq: NM_001622
• Protein Refseq: NP_001613
• MIM: 138680
• UniProt ID: P02765

Reviews

06/03/2022

Absolutely loving the lab protein – a real game changer!

10/19/2021

Great value for the quality, and the speedy delivery was a pleasant surprise!

08/16/2020

Thumbs up for the protein, it's become my new favorite.

02/27/2019

Can't help but smile at the results from the product!

12/11/2018

Balanced pricing, superior quality, and top-level customer assistance.

Q&As

How can proteomics approaches be used to study the protein-protein interactions involving AHSG in a global context?

Techniques like co-immunoprecipitation coupled with mass spectrometry can reveal AHSG's interactome, shedding light on its broader functional networks.

Explain the potential diagnostic applications of AHSG in conditions such as chronic kidney disease and diabetes?

AHSG levels may be altered in these conditions, making it a potential biomarker for disease progression or severity.

Explain the potential role of AHSG in mediating endocrine functions and its connection to insulin resistance?

AHSG's interaction with insulin and adipokines could impact insulin sensitivity and metabolism, contributing to insulin resistance in conditions like type 2 diabetes.

Discuss the interactions between AHSG and other proteins, particularly its relationship with fetuin-B and fetuin-A?

AHSG can form complexes with fetuin-B and fetuin-A, which have implications for calcium homeostasis, insulin signaling, and other physiological processes.

Explain AHSG's role in modulating immune responses and its potential relevance to autoimmune disorders?

AHSG can interact with immune cells and cytokines, potentially influencing immune responses. Dysregulated AHSG levels might contribute to autoimmune diseases.

How might AHSG's interactions with circulating proteins influence its functions and its potential clinical relevance?

AHSG's interactions with proteins like albumin and hormones can impact its bioavailability and activity, influencing physiological processes.

Describe the biochemical methods used to analyze AHSG's post-translational modifications, including glycosylation.

Techniques such as mass spectrometry, lectin affinity chromatography, and glycan analysis can provide insights into AHSG's glycosylation patterns.

How does AHSG regulate calcium homeostasis, and what implications does this have for human health?

AHSG binds to calcium ions and influences their distribution in the body. Dysregulation may impact bone health and contribute to conditions like osteoporosis.

How can researchers isolate and purify AHSG for biochemical studies in the laboratory?

Techniques such as ion exchange chromatography, gel filtration, and affinity chromatography can be used to isolate and purify AHSG from biological samples.

How might genetic variations in the AHSG gene impact its protein expression and functions?

Genetic polymorphisms in the AHSG gene might lead to altered AHSG levels or structural changes, potentially influencing its physiological roles.