| Species : |
Human |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
iFlour 488 |
| Conjugation/Label : |
1-320 aa |
| Description : |
Annexin V is a calcium-dependent phospholipid-binding protein widely used as a tool to detect apoptosis. It belongs to the annexin family of proteins, which share the ability to bind negatively charged membrane phospholipids in the presence of calcium ions. Annexin V has high affinity for phosphatidylserine (PS), a phospholipid that is normally confined to the inner leaflet of the plasma membrane in healthy cells. During early apoptosis, PS becomes externalized to the outer leaflet, where Annexin V can bind, making it a sensitive marker for programmed cell death.
Structurally, Annexin V is a ~35–36 kDa protein composed of four homologous annexin repeats that form a slightly curved, disc-like structure. Each repeat contributes to calcium-binding sites that coordinate calcium ions, enabling the protein to interact with phospholipid head groups. The convex surface of Annexin V mediates membrane binding in a calcium-dependent manner, while the opposite surface remains exposed for detection when conjugated to fluorophores or other labels. Annexin V can also form two-dimensional arrays on membrane surfaces under certain conditions. |
| Form : |
Liquid, 1×PBS buffer, pH7.4, 0.09% NaN3 with a carrier protein |
| Molecular Mass : |
This protein has a predicted molecular weight of 35.9 kDa. Under DTT-reducing conditions, the protein migrates at approximately 35 kDa on SDS-PAGE prior to conjugation. |
| Applications : |
Flow Cytometry |
| Storage : |
Briefly centrifuge the vial upon receipt. An unopened vial may be stored at 2–8 centigrade for up to six months. |
| Concentration : |
25 μg size is bottled at 0.1 mg/mL concentration. 100 μg size is bottled at lot specific concentration. |
