Recombinant Full Length Human ASL Protein, C-Flag-tagged

• Cat.No.: ASL-1888HFL
• Product Overview: Recombinant Full Length Human ASL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51.5 kDa
• AA Sequence: MASESGKLWGGRFVGAVDPIMEKFN ASIAYDRHLWEVDVQGSKAYSRGLE KAGLLTKAEMDQILHGLDKV AEEWAQGTFKLNSNDEDIHTANERR LKELIGATAGKLHTGRSRNDQVVTD LRLWMRQTCSTLSGLLWELI RTMVDRAEAERDVLFPGYTHLQRAQ PIRWSHWILSHAVALTRDSERLLEV RKRINVLPLGSGAIAGNPLG VDRELLRAELNFGAITLNSMDATSE RDFVAEFLFWASLCMTHLSRMAEDL ILYCTKEFSFVQLSDAYSTG SSLMPQKKNPDSLELIRSKAGRVFG RCAGLLMTLKGLPSTYNKDLQEDKE AVFEVSDTMSAVLQVATGVI STLQIHQENMGQALSPDMLATDLAY YLVRKGMPFRQAHEASGKAVFMAET KGVALNQLSLQELQTISPLF SGDVICVWDYGHSVEQYGALGGTAR SSVDWQIRQVRALLQAQQA SGPTRTRRLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways

Gene Information

• Gene Name: ASL argininosuccinate lyase [ Homo sapiens (human) ]
• Official Symbol: ASL
• Synonyms: ASAL
• Gene ID: 435
• mRNA Refseq: NM_000048.4
• Protein Refseq: NP_000039.2
• MIM: 608310
• UniProt ID: P04424