Recombinant Full Length Human BCAR3 Protein, C-Flag-tagged

• Cat.No.: BCAR3-1759HFL
• Product Overview: Recombinant Full Length Human BCAR3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 92.4 kDa
• AA Sequence: MAAGKFASLPRNMPVNHQFPLASSM DLLSSRSPLAEHRPDAYQDVSIHGT LPRKKKGPPPIRSCDDFSHM GTLPHSKSPRQNSPVTQDGIQESPW QDRHGETFTFRDPHLLDPTVEYVKF SKERHIMDRTPEKLKKELEE ELLLSSEDLRSHAWYHGRIPRQVSE NLVQRDGDFLVRDSLSSPGNFVLTC QWKNLAQHFKINRTVLRLSE AYSRVQYQFEMESFDSIPGLVRCYV GNRRPISQQSGAIIFQPINRTVPLR CLEEHYGTSPGQAREGSLTK GRPDVAKRLSLTMGGVQAREQNLPR GNLLRNKEKSGSQPACLDHMQDRRA LSLKAHQSESYLPIGCKLPP QSSGVDTSPCPNSPVFRTGSEPALS PAVVRRVSSDARAGEALRGSDSQLC PKPPPKPCKVPFLKVPSSPS AWLNSEANYCELNPAFATGCGRGAK LPSCAQGSHTELLTAKQNEAPGPRN SGVNYLILDDDDRERPWEPA AAQMEKGQWDKGEFVTPLLETVSSF RPNEFESKFLPPENKPLETAMLKRA KELFTNNDPKVIAQHVLSMD CRVARILGVSEEMRRNMGVSSGLEL ITLPHGHQLRLDIIERHNTMAIGIA VDILGCTGTLEDRAATLSKI IQVAVELKDSMGDLYSFSALMKALE MPQITRLEKTWTALRHQYTQTAILY EKQLKPFSKLLHEGRESTCV PPNNVSVPLLMPLVTLMERQAVTFE GTDMWEKNDQSCEIMLNHLATARFM AEAADSYRMNAERILAGFQP
  DEEMNEICKTEFQMRLLWGSKGAQV NQTERYEKFNQILTALSRKLEPPPV KQAELTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: BCAR3 BCAR3 adaptor protein, NSP family member [ Homo sapiens (human) ]
• Official Symbol: BCAR3
• Synonyms: MIG7; NSP2; AND-34; SH2D3B
• Gene ID: 8412
• mRNA Refseq: NM_003567.4
• Protein Refseq: NP_003558.1
• MIM: 604704
• UniProt ID: O75815