Recombinant Full Length Human BEST1 Protein, C-Flag-tagged

• Cat.No.: BEST1-1857HFL
• Product Overview: Recombinant Full Length Human BEST1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 67.5 kDa
• AA Sequence: MTITYTSQVANARLGSFSRLLLCWR GSIYKLLYGEFLIFLLCYYIIRFIY RLALTEEQQLMFEKLTLYCD SYIQLIPISFVLGFYVTLVVTRWWN QYENLPWPDRLMSLVSGFVEGKDEQ GRLLRRTLIRYANLGNVLIL RSVSTAVYKRFPSAQHLVQAGFMTP AEHKQLEKLSLPHNMFWVPWVWFAN LSMKAWLGGRIRDPILLQSL LNEMNTLRTQCGHLYAYDWISIPLV YTQVVTVAVYSFFLTCLVGRQFLNP AKAYPGHELDLVVPVFTFLQ FFFYVGWLKVAEQLINPFGEDDDDF ETNWIVDRNLQVSLLAVDEMHQDLP RMEPDMYWNKPEPQPPYTAA SAQFRRASFMGSTFNISLNKEEMEF QPNQEDEEDAHAGIIGRFLGLQSHD HHPPRANSRTKLLWPKRESL LHEGLPKNHKAAKQNVRGQEDNKAW KLKAVDAFKSAPLYQRPGYYSAPQT PLSPTPMFFPLEPSAPSKLH SVTGIDTKDKSLKTVSSGAKKSFEL LSESDGALMEHPEVSQVRRKTVEFN LTDMPEIPENHLKEPLEQSP TNIHTTLKDHMDPYWALENRDEAHS TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane

Gene Information

• Gene Name: BEST1 bestrophin 1 [ Homo sapiens (human) ]
• Official Symbol: BEST1
• Synonyms: ARB; BMD; BEST; RP50; VMD2; TU15B; Best1V1Delta2
• Gene ID: 7439
• mRNA Refseq: NM_004183.4
• Protein Refseq: NP_004174.1
• MIM: 607854
• UniProt ID: O76090