| Description : |
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. |
| Source : |
In Vitro Cell Free System |
| Species : |
Human |
| Tag : |
GST |
| Form : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
| Molecular Mass : |
61.38 kDa |
| Protein Length : |
324 amino acids |
| AA Sequence : |
MTAAGHHAEVVVDPEDERAIEMFMN KNPPARRTLADIIMEKLTEKQTEVE TVMSEVSGFPMPQLDPRVLEVYRGV REVLSKYRSGKLPKAFKIIPALSNW DQILYVTEPEAWTAAAMYQATRIFA SNLKERMAQRFYNLVLLPRVRDDVA EYKRLNFHLYMALKKALFKPGAWFK GILIPLCESGTCTLREAIIVGSIIT KCSIPVLHSSAAMLKIAEMEYSGAN SIFLRLLLDKKYALPYRVLDALVFH FLGFRTEKRELPVLWHQCLLTLVQR YKADLATDQKEALLELLRLQPHPQL SPEIRRELQSAVPRDVEDVPITVE |
| Quality Control Test : |
12.5% SDS-PAGE Stained with Coomassie Blue. |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
