Recombinant Full Length Human CBX5 Protein, C-Flag-tagged

• Cat.No.: CBX5-2036HFL
• Product Overview: Recombinant Full Length Human CBX5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 22 kDa
• AA Sequence: MGKKTKRTADSSSSEDEEEYVVEKV LDRRVVKGQVEYLLKWKGFSEEHNT WEPEKNLDCPELISEFMKKY KKMKEGENNKPREKSESNKRKSNFS NSADDIKSKKKREQSNDIARGFERG LEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKCPQIVI AFYEERLTWHAYPEDAENKEKETAK S TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: CBX5 chromobox 5 [ Homo sapiens (human) ]
• Official Symbol: CBX5
• Synonyms: HP1; HP1A; HEL25
• Gene ID: 23468
• mRNA Refseq: NM_012117.3
• Protein Refseq: NP_036249.1
• MIM: 604478
• UniProt ID: P45973