Recombinant Full Length Human CCT5 Protein, C-Flag-tagged

• Cat.No.: CCT5-862HFL
• Product Overview: Recombinant Full Length Human CCT5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 59.5 kDa
• AA Sequence: MASMGTLAFDEYGRPFLIIKDQDRK SRLMGLEALKSHIMAAKAVANTMRT SLGPNGLDKMMVDKDGDVTV TNDGATILSMMDVDHQIAKLMVELS KSQDDEIGDGTTGVVVLAGALLEEA EQLLDRGIHPIRIADGYEQA ARVAIEHLDKISDSVLVDIKDTEPL IQTAKTTLGSKVVNSCHRQMAEIAV NAVLTVADMERRDVDFELIK VEGKVGGRLEDTKLIKGVIVDKDFS HPQMPKKVEDAKIAILTCPFEPPKP KTKHKLDVTSVEDYKALQKY EKEKFEEMIQQIKETGANLAICQWG FDDEANHLLLQNNLPAVRWVGGPEI ELIAIATGGRIVPRFSELTA EKLGFAGLVQEISFGTTKDKMLVIE QCKNSRAVTIFIRGGNKMIIEEAKR SLHDALCVIRNLIRDNRVVY GGGAAEISCALAVSQEADKCPTLEQ YAMRAFADALEVIPMALSENSGMNP IQTMTEVRARQVKEMNPALG
  IDCLHKGTNDMKQQHVIETLIGKKQ QISLATQMVRMILKIDDIRKPGESE ETRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: CCT5 chaperonin containing TCP1 subunit 5 [ Homo sapiens (human) ]
• Official Symbol: CCT5
• Synonyms: CCTE; HEL-S-69; PNAS-102; CCT-epsilon; TCP-1-epsilon
• Gene ID: 22948
• mRNA Refseq: NM_012073.5
• Protein Refseq: NP_036205.1
• MIM: 610150
• UniProt ID: P48643