Recombinant Full Length Human CENPA Protein, C-Flag-tagged

• Cat.No.: CENPA-572HFL
• Product Overview: Recombinant Full Length Human CENPA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 15.8 kDa
• AA Sequence: MGPRRRSRKPEAPRRRSPSPTPTPG PSRRGPSLGASSHQHSRRRQGWLKE IRKLQKSTHLLIRKLPFSRL AREICVKFTRGVDFNWQAQALLALQ EAAEAFLVHLFEDAYLLTLHAGRVT LFPKDVQLARRIRGLEEGLGTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: CENPA centromere protein A [ Homo sapiens (human) ]
• Official Symbol: CENPA
• Synonyms: CenH3; CENP-A
• Gene ID: 1058
• mRNA Refseq: NM_001809.4
• Protein Refseq: NP_001800.1
• MIM: 117139
• UniProt ID: P49450