Recombinant Full Length Human cereblon Protein, GST tagged

• Cat.No.: CRBN-09HFL
• Product Overview: Recombinant full-length human CRBN was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-442 aa
• Description: This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
• Tag: N-GST
• Molecular Mass: ~78 kDa
• AA Sequence: MAGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENKGEDAPLDQGSGAILASTFSNSGSYFALTDDSKRLILFRTKPWQCLSVRTVARRCTALTFIASEEKVLVADKSGDVYSFSVLEPHGCGRLELGHLSMLLDVAVSPDDRFILTADRDEKIRVSWAAAPHSIESFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAPQKFAASRIAFWCQENCVALLCDGTSVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQGLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSAGADASFSSLYKATFDNVTSYLKKKEERLQQQLEKKQRRQSPPPGPDGHAKKMRPGEATLSCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 70% by densitometry.
• Stability: One year at –70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
• Concentration: 0.05 μg/μL
• Shipping: Product shipped on dry ice.
• Reference: 1. Ito, T., et al. (2010). Identification of a primary target of thalidomide teratogenicity. Science, 327(5971), 1345-1350.
  2. Krönke, J., et al. (2014). Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS. Nature, 523(7562), 183-188.
  3. Higgins, J. J., et al. (2004). A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation. Neurology, 63(10), 1927-1931.

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens (human) ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715
• Gene ID: 51185
• mRNA Refseq: NM_016302
• Protein Refseq: NP_057386
• MIM: 609262
• UniProt ID: Q96SW2