Recombinant Full Length Human CFI Protein, C-Flag-tagged

• Cat.No.: CFI-1531HFL
• Product Overview: Recombinant Full Length Human CFI Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 63.4 kDa
• AA Sequence: MKLLHVFLLFLCFHLRFCKVTYTSQ EDLVEKKCLAKKYTHLSCDKVFCQP WQRCIEGTCVCKLPYQCPKN GTAVCATNRRSFPTYCQQKSLECLH PGTKFLNNGTCTAEGKFSVSLKHGN TDSEGIVEVKLVDQDKTMFI CKSSWSMREANVACLDLGFQQGADT QRRFKLSDLSINSTECLHVHCRGLE TSLAECTFTKRRTMGYQDFA DVVCYTQKADSPMDDFFQCVNGKYI SQMKACDGINDCGDQSDELCCKACQ GKGFHCKSGVCIPSQYQCNG EVDCITGEDEVGCAGFASVAQEETE ILTADMDAERRRIKSLLPKLSCGVK NRMHIRRKRIVGGKRAQLGD LPWQVAIKDASGITCGGIYIGGCWI LTAAHCLRASKTHRYQIWTTVVDWI HPDLKRIVIEYVDRIIFHEN YNAGTYQNDIALIEMKKDGNKKDCE LPRSIPACVPWSPYLFQPNDTCIVS GWGREKDNERVFSLQWGEVK LISNCSKFYGNRFYEKEMECAGTYD GSIDACKGDSGGPLVCMDANNVTYV WGVVSWGENCGKPEFPGVYT
  KVANYFDWISYHVGRPFISQYNVTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades

Gene Information

• Gene Name: CFI complement factor I [ Homo sapiens (human) ]
• Official Symbol: CFI
• Synonyms: FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
• Gene ID: 3426
• mRNA Refseq: NM_000204.5
• Protein Refseq: NP_000195.3
• MIM: 217030
• UniProt ID: P05156