Recombinant Full Length Human CKB Protein, C-Flag-tagged

• Cat.No.: CKB-1210HFL
• Product Overview: Recombinant Full Length Human CKB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.5 kDa
• AA Sequence: MPFSNSHNALKLRFPAEDEFPDLSA HNNHMAKVLTPELYAELRAKSTPSG FTLDDVIQTGVDNPGHPYIM TVGCVAGDEESYEVFKDLFDPIIED RHGGYKPSDEHKTDLNPDNLQGGDD LDPNYVLSSRVRTGRSIRGF CLPPHCSRGERRAIEKLAVEALSSL DGDLAGRYYALKSMTEAEQQQLIDD HFLFDKPVSPLLLASGMARD WPDARGIWHNDNKTFLVWVNEEDHL RVISMQKGGNMKEVFTRFCTGLTQI ETLFKSKDYEFMWNPHLGYI LTCPSNLGTGLRAGVHIKLPNLGKH EKFSEVLKRLRLQKRGTGGVDTAAV GGVFDVSNADRLGFSEVELV
  QMVVDGVKLLIEMEQRLEQGQAIDD LMPAQKTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways

Gene Information

• Gene Name: CKB creatine kinase B [ Homo sapiens (human) ]
• Official Symbol: CKB
• Synonyms: BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• Gene ID: 1152
• mRNA Refseq: NM_001823.5
• Protein Refseq: NP_001814.2
• MIM: 123280
• UniProt ID: P12277