Recombinant Full Length Human COL15A1 Protein, C-Flag-tagged

• Cat.No.: COL15A1-277HFL
• Product Overview: Recombinant Full Length Human COL15A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 138.9 kDa
• AA Sequence: MAPRRNNGQCWCLLMLLSVSTPLPA VTQTRGATETASQGHLDLTQLIGVP LPSSVSFVTGYGGFPAYSFG PGANVGRPARTLIPSTFFRDFAISV VVKPSSTRGGVLFAITDAFQKVIYL GLRLSGVEDGHQRIILYYTE PGSHVSQEAAAFSVPVMTHRWNRFA MIVQGEEVTLLVNCEEHSRIPFQRS SQALAFESSAGIFMGNAGAT GLERFTGSLQQLTVHPDPRTPEELC DPEESSASGETSGLQEADGVAEILE AVTYTQASPKEAKVEPINTP PTPSSPFEDMELSGEPVPEGTLETT NMSIIQHSSPKQGSGEILNDTLEGV HSVDGDPITDSGSGAGAFLD IAEEKNLAATAAGLAEVPISTAGEA EASSVPTGGPTLSMSTENPEEGVTP GPDNEERLAATAAGEAEALA SMPGEVEASGVAPGELDLSMSAQSL GEEATVGPSSEDSLTTAAAATEVSL STFEDEEASGVPTDGLAPLT ATMAPERAVTSGPGDEEDLAAATTE EPLITAGGEESGSPPPDGPPLPLPT VAPERWITPAQREHVGMKGQ AGPKGEKGDAGEELPGPPEPSGPVG PTAGAEAEGSGLGWGSDVGSGSGDL VGSEQLLRGPPGPPGPPGLP GIPGKPGTDVFMGPPGSPGEDGPAG EPGPPGPEGQPGVDGATGLPGMKGE KGARGPNGSVGEKGDPGNRG LPGPPGKKGQAGPPGVMGPPGPPGP PGPPGPGCTMGLGFEDTEGSGSTQL LNEPKLSRPTAAIGLKGEKG DRGPKGERGMDGASIVGPPGPRGPP GHIKVLSNSLINITHGFMNFSDIPE LVGPPGPDGLPGLPGFPGPR GPKGDTGLPGFPGLKGEQGEKGEPG AILTEDIPLERLMGKKGEPGMHGAP GPMGPKGPPGHKGEFGLPGR PGRPGLNGLKGTKGDPGVIMQGPPG LPGPPGPPGPPGAVINIKGAIFPIP VRPHCKMPVDTAHPGSPELI TFHGVKGEKGSWGLPGSKGEKGDQG AQGPPGPPLDLAYLRHFLNNLKGEN GDKGFKGEKGEKGDINGSFL MSGPPGLPGNPGPAGQKGETVVGPQ GPPGAPGLPGPPGFGRPGDPGPPGP PGPPGPPAILGAAVALPGPP GPPGQPGLPGSRNLVTAFSNMDDML QKAHLVIEGTFIYLRDSTEFFIRVR DGWKKLQLGELIPIPADSPP PPALSSNPHQLLPPPNPISSANYEK PALHLAALNMPFSGDIRADFQCFKQ ARAAGLLSTYRAFLSSHLQD LSTIVRKAERYSLPIVNLKGQVLFN NWDSIFSGHGGQFNMHIPIYSFDGR DIMTDPSWPQKVIWHGSSPH
  GVRLVDNYCEAWRTADTAVTGLASP LSTGKILDQKAYSCANRLIVLCIEN SFMTDARKTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: COL15A1 collagen type XV alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL15A1
• Synonyms: FLJ38566
• Gene ID: 1306
• mRNA Refseq: NM_001855.5
• Protein Refseq: NP_001846.3
• MIM: 120325
• UniProt ID: P39059