Recombinant Full Length Human CPSF3 Protein, C-Flag-tagged

• Cat.No.: CPSF3-699HFL
• Product Overview: Recombinant Full Length Human CPSF3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 77.3 kDa
• AA Sequence: MSAIPAEESDQLLIRPLGAGQEVGR SCIILEFKGRKIMLDCGIHPGLEGM DALPYIDLIDPAEIDLLLIS HFHLDHCGALPWFLQKTSFKGRTFM THATKAIYRWLLSDYVKVSNISADD MLYTETDLEESMDKIETINF HEVKEVAGIKFWCYHAGHVLGAAMF MIEIAGVKLLYTGDFSRQEDRHLMA AEIPNIKPDILIIESTYGTH IHEKREEREARFCNTVHDIVNRGGR GLIPVFALGRAQELLLILDEYWQNH PELHDIPIYYASSLAKKCMA VYQTYVNAMNDKIRKQININNPFVF KHISNLKSMDHFDDIGPSVVMASPG MMQSGLSRELFESWCTDKRN GVIIAGYCVEGTLAKHIMSEPEEIT TMSGQKLPLKMSVDYISFSAHTDYQ QTSEFIRALKPPHVILVHGE QNEMARLKAALIREYEDNDEVHIEV HNPRNTEAVTLNFRGEKLAKVMGFL ADKKPEQGQRVSGILVKRNF NYHILSPCDLSNYTDLAMSTVKQTQ AIPYTGPFNLLCYQLQKLTGDVEEL EIQEKPALKVFKNITVIQEP GMVVLEWLANPSNDMYADTVTTVIL EVQSNPKIRKGAVQKVSKKLEMHVY SKRLEIMLQDIFGEDCVSVK
  DDSILSVTVDGKTANLNLETRTVEC EEGSEDDESLREMVELAAQRLYEAL TPVHTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: CPSF3 cleavage and polyadenylation specific factor 3 [ Homo sapiens (human) ]
• Official Symbol: CPSF3
• Synonyms: CPSF73; NEDMHS; CPSF-73; NEDMHSN
• Gene ID: 51692
• mRNA Refseq: NM_016207.4
• Protein Refseq: NP_057291.1
• MIM: 606029
• UniProt ID: Q9UKF6