Recombinant Full Length Human CTSL Protein, C-Flag-tagged

• Cat.No.: CTSL-1680HFL
• Product Overview: Recombinant Full Length Human CTSL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.8 kDa
• AA Sequence: MNPTLILAAFCLGIASATLTFDHSL EAQWTKWKAMHNRLYGMNEEGWRRA VWEKNVKMIELHNQEYREGK HSFTMAMNAFGDMTSEEFRQVMNGF QNRKPRKGKVFQEPLFYEAPRSVDW REKGYVTPVKNQGQCGSCWA FSATGALEGQMFRKTGRLISLSEQN LVDCSGPQGNEGCNGGLMDYAFQYV QDNGGLDSEESYPYEATEES CKYNPKYSVANDTGFVDIPKQEKAL MKAVATVGPISVAIDAGHESFLFYK EGIYFEPDCSSEDMDHGVLV
  VGYGFESTESDNNKYWLVKNSWGEE WGMGGYVKMAKDRRNHCGIASAASY PTVTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome

Gene Information

• Gene Name: CTSL cathepsin L [ Homo sapiens (human) ]
• Official Symbol: CTSL
• Synonyms: MEP; CATL; CTSL1
• Gene ID: 1514
• mRNA Refseq: NM_001912.5
• Protein Refseq: NP_001903.1
• MIM: 116880
• UniProt ID: P07711