Recombinant Full Length Human CUL2 Protein, C-Flag-tagged

• Cat.No.: CUL2-1685HFL
• Product Overview: Recombinant Full Length Human CUL2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within protein catabolic process. Located in nucleoplasm. Part of Cul2-RING ubiquitin ligase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 86.8 kDa
• AA Sequence: MSLKPRVVDFDETWNKLLTTIKAVV MLEYVERATWNDRFSDIYALCVAYP EPLGERLYTETKIFLENHVR HLHKRVLESEEQVLVMYHRYWEEYS KGADYMDCLYRYLNTQFIKKNKLTE ADLQYGYGGVDMNEPLMEIG ELALDMWRKLMVEPLQAILIRMLLR EIKNDRGGEDPNQKVIHGVINSFVH VEQYKKKFPLKFYQEIFESP FLTETGEYYKQEASNLLQESNCSQY MEKVLGRLKDEEIRCRKYLHPSSYT KVIHECQQRMVADHLQFLHA ECHNIIRQEKKNDMANMYVLLRAVS TGLPHMIQELQNHIHDEGLRATSNL TQENMPTLFVESVLEVHGKF VQLINTVLNGDQHFMSALDKALTSV VNYREPKSVCKAPELLAKYCDNLLK KSAKGMTENEVEDRLTSFIT VFKYIDDKDVFQKFYARMLAKRLIH GLSMSMDSEEAMINKLKQACGYEFT SKLHRMYTDMSVSADLNNKF NNFIKNQDTVIDLGISFQIYVLQAG AWPLTQAPSSTFAIPQELEKSVQMF ELFYSQHFSGRKLTWLHYLC TGEVKMNYLGKPYVAMVTTYQMAVL LAFNNSETVSYKELQDSTQMNEKEL TKTIKSLLDVKMINHDSEKE DIDAESSFSLNMNFSSKRTKFKITT SMQKDTPQEMEQTRSAVDEDRKMYL QAAIVRIMKARKVLRHNALI
  QEVISQSRARFNPSISMIKKCIEVL IDKQYIERSQASADEYSYVATRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: CUL2 cullin 2 [ Homo sapiens (human) ]
• Official Symbol: CUL2
• Synonyms: MGC131970
• Gene ID: 8453
• mRNA Refseq: NM_003591.4
• Protein Refseq: NP_003582.2
• MIM: 603135
• UniProt ID: Q13617