Recombinant Full Length Human CYP2B6 Protein, C-Flag-tagged

• Cat.No.: CYP2B6-1884HFL
• Product Overview: Recombinant Full Length Human CYP2B6 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.1 kDa
• AA Sequence: MELSVLLFLALLTGLLLLLVQCHPN THDRLPPGPRPLPLLGNLLQMDRRG LLKSFLRFREKYGDVFTVHL GPRPVVMLCGVEAIREALVDKAEAF SGRGKIAMVDPFFRGYGVIFANGNR WKVLRRFSVTTMRDFGMGKR SVEERIQEEAQCLIEELRKSKGALM DPTFLFQSITANIICSIVFGKRFHY QDQEFLKMLNLFYQTFSLIS SVFGQLFELFSGFLKYFPGAHRQVY KNLQEINAYIGHSVEKHRETLDPSA PKDLIDTYLLHMEKEKSNAH SEFSHQNLNLNTLSLFFAGTETTST TLRYGFLLMLKYPHVAERVYREIEQ VIGPHRPPELHDRAKMPYTE AVIYEIQRFSDLLPMGVPHIVTQHT SFRGYIIPKDTEVFLILSTALHDPH YFEKPDAFNPDHFLDANGAL KKTEAFIPFSLGKRICLGEGIARAE LFLFFTTILQNFSMASPVAPEDIDL TPQECGVGKIPPTYQIRFLP R TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism

Gene Information

• Gene Name: CYP2B6 cytochrome P450 family 2 subfamily B member 6 [ Homo sapiens (human) ]
• Official Symbol: CYP2B6
• Synonyms: CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
• Gene ID: 1555
• mRNA Refseq: NM_000767.5
• Protein Refseq: NP_000758.1
• MIM: 123930
• UniProt ID: P20813