Recombinant Full Length Human CYP51A1 Protein, C-Flag-tagged

• Cat.No.: CYP51A1-1748HFL
• Product Overview: Recombinant Full Length Human CYP51A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57.1 kDa
• AA Sequence: MAAAAGMLLLGLLQAGGSVLGQAME KVTGGNLLSMLLIACAFTLSLVYLI RLAAGHLVQLPAGVKSPPYI FSPIPFLGHAIAFGKSPIEFLENAY EKYGPVFSFTMVGKTFTYLLGSDAA ALLFNSKNEDLNAEDVYSRL TTPVFGKGVAYDVPNPVFLEQKKML KSGLNIAHFKQHVSIIEKETKEYFE SWGESGEKNVFEALSELIIL TASHCLHGKEIRSQLNEKVAQLYAD LDGGFSHAAWLLPGWLPLPSFRRRD RAHREIKDIFYKAIQKRRQS QEKIDDILQTLLDATYKDGRPLTDD EVAGMLIGLLLAGQHTSSTTSAWMG FFLARDKTLQKKCYLEQKTV CGENLPPLTYDQLKDLNLLDRCIKE TLRLRPPVMIMMRMARTPQTVAGYT IPPGHQVCVSPTVNQRLKDS WVERLDFNPDRYLQDNPASGEKFAY VPFGAGRHRCIGENFAYVQIKTIWS TMLRLYEFDLIDGYFPTVNY
  TTMIHTPENPVIRYKRRSKTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis

Gene Information

• Gene Name: CYP51A1 cytochrome P450 family 51 subfamily A member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP51A1
• Synonyms: LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
• Gene ID: 1595
• mRNA Refseq: NM_000786.4
• Protein Refseq: NP_000777.1
• MIM: 601637
• UniProt ID: Q16850