Recombinant Full Length Human DAZAP1 Protein, C-Flag-tagged

• Cat.No.: DAZAP1-1698HFL
• Product Overview: Recombinant Full Length Human DAZAP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 43.2 kDa
• AA Sequence: MNNSGADEIGKLFVGGLDWSTTQET LRSYFSQYGEVVDCVIMKDKTTNQS RGFGFVKFKDPNCVGTVLAS RPHTLDGRNIDPKPCTPRGMQPERT RPKEGWQKGPRSDNSKSNKIFVGGI PHNCGETELREYFKKFGVVT EVVMIYDAEKQRPRGFGFITFEDEQ SVDQAVNMHFHDIMGKKVEVKRAEP RDSKSQAPGQPGASQWGSRV VPNAANGWAGQPPPTWQQGYGPQGM WVPAGQAIGGYGPPPAGRGAPPPPP PFTSYIVSTPPGGFPPPQGF PQGYGAPPQFSFGYGPPPPPPDQFA PPGVPPPPATPGAAPLAFPPPPSQA APDMSKPPTAQPDFPYGQYA
  GYGQDLSGFGQGFSDPSQQPPSYGG PSVPGSGGPPAGGSGFGRGQNHNVQ GFHPYRRTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Stem cell - Pluripotency

Gene Information

• Gene Name: DAZAP1 DAZ associated protein 1 [ Homo sapiens (human) ]
• Official Symbol: DAZAP1
• Synonyms: MGC19907
• Gene ID: 26528
• mRNA Refseq: NM_018959.4
• Protein Refseq: NP_061832.2
• MIM: 607430
• UniProt ID: Q96EP5