Recombinant Full Length Human DBI Protein, C-Flag-tagged

• Cat.No.: DBI-1107HFL
• Product Overview: Recombinant Full Length Human DBI Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 11.6 kDa
• AA Sequence: MWGDLWLLPPASANPGTGTEAEFEK AAEEVRHLKTKPSDEEMLFIYGHYK QATVGDINTERPGMLDFTGK
  AKWDAWNELKGTSKEDAMKAYINKV EELKKKYGITRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: PPAR signaling pathway

Gene Information

• Gene Name: DBI diazepam binding inhibitor, acyl-CoA binding protein [ Homo sapiens (human) ]
• Official Symbol: DBI
• Synonyms: EP; ACBP; ACBD1; CCK-RP
• Gene ID: 1622
• mRNA Refseq: NM_020548.9
• Protein Refseq: NP_065438.1
• MIM: 125950
• UniProt ID: P07108