Recombinant Full Length Human DCLK1 Protein, C-Flag-tagged

• Cat.No.: DCLK1-723HFL
• Product Overview: Recombinant Full Length Human DCLK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 80.9 kDa
• AA Sequence: MSFGRDMELEHFDERDKAQRYSRGS RVNGLPSPTHSAHCSFYRTRTLQTL SSEKKAKKVRFYRNGDRYFK GIVYAISPDRFRSFEALLADLTRTL SDNVNLPQGVRTIYTIDGLKKISSL DQLVEGESYVCGSIEPFKKL EYTKNVNPNWSVNVKTTSASRAVSS LATAKGSPSEVRENKDFIRPKLVTI IRSGVKPRKAVRILLNKKTA HSFEQVLTDITDAIKLDSGVVKRLY TLDGKQVMCLQDFFGDDDIFIACGP EKFRYQDDFLLDESECRVVK STSYTKIASSSRRSTTKSPGPSRRS KSPASTSSVNGTPGSQLSTPRSGKS PSPSPTSPGSLRKQRSSQHG GSSTSLASTKVCSSMDENDGPGEEV SEEGFQIPATITERYKVGRTIGDGN FAVVKECVERSTAREYALKI IKKSKCRGKEHMIQNEVSILRRVKH PNIVLLIEEMDVPTELYLVMELVKG GDLFDAITSTNKYTERDASG MLYNLASAIKYLHSLNIVHRDIKPE NLLVYEHQDGSKSLKLGDFGLATIV DGPLYTVCGTPTYVAPEIIA ETGYGLKVDIWAAGVITYILLCGFP PFRGSGDDQEVLFDQILMGQVDFPS PYWDNVSDSAKELITMMLLV DVDQRFSAVQVLEHPWVNDDGLPEN EHQLSVAGKIKKHFNTGPKPNSTAA GVSVIALDHGFTIKRSGSLD
  YYQQPGMYWIRPPLLIRRGRFSDED ATRMTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: DCLK1 doublecortin like kinase 1 [ Homo sapiens (human) ]
• Official Symbol: DCLK1
• Synonyms: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
• Gene ID: 9201
• mRNA Refseq: NM_004734.5
• Protein Refseq: NP_004725.1
• MIM: 604742
• UniProt ID: O15075