Recombinant Full Length Human DDX39B Protein, C-Flag-tagged

• Cat.No.: DDX39B-2024HFL
• Product Overview: Recombinant Full Length Human DDX39B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 48.8 kDa
• AA Sequence: MAENDVDNELLDYEDDEVETAAGGD GAEAPAKKDVKGSYVSIHSSGFRDF LLKPELLRAIVDCGFEHPSE VQHECIPQAILGMDVLCQAKSGMGK TAVFVLATLQQLEPVTGQVSVLVMC HTRELAFQISKEYERFSKYM PNVKVAVFFGGLSIKKDEEVLKKNC PHIVVGTPGRILALARNKSLNLKHI KHFILDECDKMLEQLDMRRD VQEIFRMTPHEKQVMMFSATLSKEI RPVCRKFMQDPMEIFVDDETKLTLH GLQQYYVKLKDNEKNRKLFD LLDVLEFNQVVIFVKSVQRCIALAQ LLVEQNFPAIAIHRGMPQEERLSRY QQFKDFQRRILVATNLFGRG MDIERVNIAFNYDMPEDSDTYLHRV ARAGRFGTKGLAITFVSDENDAKIL NDVQDRFEVNISELPDEIDI SSYIEQTR TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Spliceosome

Gene Information

• Gene Name: DDX39B DExD-box helicase 39B [ Homo sapiens (human) ]
• Official Symbol: DDX39B
• Synonyms: BAT1; UAP56; D6S81E
• Gene ID: 7919
• mRNA Refseq: NM_004640.7
• Protein Refseq: NP_004631.1
• MIM: 142560
• UniProt ID: Q13838