Recombinant Full Length Human DGCR8 Protein, C-Flag-tagged

• Cat.No.: DGCR8-1141HFL
• Product Overview: Recombinant Full Length Human DGCR8 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 85.9 kDa
• AA Sequence: METDESPSPLPCGPAGEAVMESRAR PFQALPREQSPPPPLQTSSGAEVMD VGSGGDGQSELPAEDPFNFY GASLLSKGSFSKGRLLIDPNCSGHS PRTARHAPAVRKFSPDLKLLKDVKI SVSFTESCRSKDRKVLYTGA ERDVRAECGLLLSPVSGDVHACPFG GSVGDGVGIGGESADKKDEENELDQ EKRVEYAVLDELEDFTDNLE LDEEGAGGFTAKAIVQRDRVDEEAL NFPYEDDFDNDVDALLEEGLCAPKK RRTEEKYGGDSDHPSDGETS VQPMMTKIKTVLKSRGRPPTEPLPD GWIMTFHNSGVPVYLHRESRVVTWS RPYFLGTGSIRKHDPPLSSI PCLHYKKMKDNEEREQSSDLTPSGD VSPVKPLSRSAELEFPLDEPDSMGA DPGPPDEKDPLGAEAAPGAL GQVKAKVEVCKDESVDLEEFRSYLE KRFDFEQVTVKKFRTWAERRQFNRE MKRKQAESERPILPANQKLI TLSVQDAPTKKEFVINPNGKSEVCI LHEYMQRVLKVRPVYNFFECENPSE PFGASVTIDGVTYGSGTASS KKLAKNKAARATLEILIPDFVKQTS EEKPKDSEELEYFNHISIEDSRVYE LTSKAGLLSPYQILHECLKR NHGMGDTSIKFEVVPGKNQKSEYVM ACGKHTVRGWCKNKRVGKQLASQKI LQLLHPHVKNWGSLLRMYGR ESSKMVKQETSDKSVIELQQYAKKN KPNLHILSKLQEEMKRLAEEREETR KKPKMSIVASAQPGGEPLCT
  VDVTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DGCR8 DGCR8 microprocessor complex subunit [ Homo sapiens (human) ]
• Official Symbol: DGCR8
• Synonyms: Gy1; pasha; DGCRK6; C22orf12
• Gene ID: 54487
• mRNA Refseq: NM_022720.7
• Protein Refseq: NP_073557.3
• MIM: 609030
• UniProt ID: Q8WYQ5